孕晚期孕妇肠道菌群与孕期体重增长的相关性

Association between maternal gestational weight gain and gut microbiota at late pregnancy

摘要:

【背景】孕妇体重过度增长在全球范围内呈现上升趋势，对母亲和子代健康均会造成不良影响。肠道菌群与人体众多疾病相关，其有可能作为重要的调节因素影响母婴健康。【目的】研究孕晚期孕妇肠道菌群的多样性和群落组成，探讨孕期体重增长对肠道菌群的影响。【方法】收集62位孕晚期(36.82±2.19孕周)孕妇粪便样本，利用Miseq高通量测序技术对样品中16S rRNA基因V3?V4区序列进行测序，分析不同孕期体重增长水平的孕妇肠道菌群的多样性、群落结构和物种丰度，探讨孕期体重增长对孕妇肠道菌群的影响。【结果】与正常孕妇相比，孕期体重增长过度孕妇肠道菌群多样性显著降低，群落结构变化不大；在菌群组成上，筛选出5个与孕期体重增长最为相关的菌群，分别为Alistipes spp.、Eubacterium-nodatum group spp.、Oxalobacter spp.、Raoultella spp.和Odoribacter spp.，其中Alistipes spp.不仅是相关程度最高也是丰度最高的菌群，与孕期体重增长密切相关。【结论】孕期体重增长对孕晚期孕妇肠道菌群群落结构影响显著，在孕期保持肠道菌群稳态能够促进母婴健康。

Abstract:

[Background] Excessive gestational weight gain among women has increased dramatically worldwide, which is associated with maternal and infants’ health. Recent evidence supports that gut microbiota may be an important regulation factor correlated with numerous human diseases. [Objective] To explore maternal gut microbiota diversity and composition at late pregnancy and changes of maternal gut microbiota with different gestational weight gain status, evaluating the effect of gestational weight gain on maternal gut microbiota. [Methods] we collected 62 pregnant women’s fecal samples at late pregnancy (36.82±2.19 gestation weeks), using high-throughput Miseq sequencing technology for sequencing 16S rRNA gene sequence in the V3?V4 region of samples. [Results] maternal gut microbiota of excessive gestational weight gain has a significantly lower diversity. The most important five genus of gestational weight gain are Alistipes spp., Eubacterium-nodatum group spp., Oxalobacter spp., Raoultella spp. and Odoribacter spp. And the intensity of correlation of Alistipes spp. is the most strong and abundant. There are no differences between adequate and excessive groups in gut microbiota structure. [Conclusion] Gestational weight gain can affect maternal gut microbiota at late pregnancy and the balance of gut microbiota will stimulate a healthy development of maternal and infants.