miR-34b调控肠道病毒71型在人横纹肌肉瘤细胞中的复制及机制

miR-34b regulates the replication of Enterovirus 71 in rhabdomyosarcoma cell

摘要:

【背景】研究发现microRNAs (miRNAs)可以参与调控病毒在宿主细胞内感染和复制的过程。【目的】研究miR-34b对肠道病毒71型(Enterovirus 71，EV71)在宿主细胞内的复制及其可能机制。【方法】在人横纹肌肉瘤(Rhabdomyosarcoma，RD)细胞中转染miR-34b mimics和Inhibitor，通过Western blot和real-time PCR实验检验EV71病毒的复制和表达情况。随后利用双荧光素酶报告系统验证miR-34b与潜在靶点eIF4E的相互作用，并检测miR-34b对RD细胞中eIF4E mRNA表达水平的影响。【结果】miR-34b可以促进病毒在RD细胞中的复制和表达，而miR-34b抑制剂有抑制病毒复制的作用，细胞内miR-34b可以通过作用于靶基因eIF4E调控EV71在宿主细胞中的复制过程。【结论】揭示了miR-34b在EV71病毒复制过程中的调控作用及机制，研究EV71病毒与宿主miRNAs的相互作用机制为进一步阐明EV71病毒感染与复制机理奠定了基础。

Abstract:

[Background] MicroRNAs (miRNAs) play an important role in the process of infection and replication of virus in host cells. [Objective] In this study, we examined miR-34b effect on the replication of Enterovirus 71 in RD (rhabdomyosarcoma) cells. [Methods] Western blot and real-time PCR test were performed to investigate the effect of miRNAs on viral replication. Furthermore, dual-luciferase reporter assay was used to detect the interaction between miR-34b and potential target eIF4E, and the expression of eIF4E at mRNA levels was tested by real-time PCR. [Results] The study showed that miR-34b can stimulate the replication of EV71 virus. On the contrary, miR-34b inhibitor can suppress EV71 virus replication. Cellular miR-34b can regulate the replication of EV71 virus through binding to eIF4E in host cells. [Conclusion] Our paper should report the role of miR-34b in this regulation for the first time. Our findings support the notion that the cellular miRNAs play an important role in the host and virus infection.