共生真菌Simplicillium lanosoniveum促进衣藻生长和脂类合成

Enhancing cell growth and lipid production of Chlamydomonas reinhardtii by co-culturing with the fungus Simplicillium lanosoniveum

摘要:

【背景】藻类是生产生物柴油的主要原料，而一些真菌和细菌能够与藻类共生并提高生物柴油产量，因此藻-菌共生培养技术成为国内外研究的热点。【目的】研究共生真菌Simplicillium lanosoniveum对衣藻Chlamydomonas reinhardtii细胞生长和脂类合成的影响。【方法】将分离的蓝藻共生真菌和衣藻混合(共生)培养。【结果】与衣藻单独培养相比，混合培养衣藻的比生长速率(0.20 d?1)、细胞产率[0.17 g/(L·d)]和生物量(2.85 g/L)分别提高了10.3%、51.3%和55.7%；脂类比合成速率[0.68 mg/(g·d)]、合成速率[1.95 mg/(L·d)]和含量(220.4 mg/g)分别提高了33.3%、107.5%和32.0%，并且脂类中的饱和脂肪酸以及单不饱和脂肪酸C18-1和C18-2的比例上升，有利于生物柴油的加工。【结论】真菌Simplicillium lanosoniveum能够促进衣藻的生长和脂类合成，因此藻-菌混合培养可用于生物柴油原料的生产。

Abstract:

[Background] Algae are the major feed-stocks for biofuel production, and some fungi and bacteria can establish symbiotic relationships with algae and promote their productivities. Hence, co-culture of algae with fungi or bacteria becomes the research hotspot worldwide. [Objective] To study the effects of cyanobacterium-symbiotic fungus Simplicillium lanosoniveum on the cell growth and lipid synthesis of Chlamydomonas reinhardtii. [Methods] C. reinhardtii was co-cultured with Simplicillium lanosoniveum. [Results] Compared to C. reinhardtii monoculture, the specific growth rate (0.20 d?1), cell productivity (0.17 g/(L·d)) and biomass (2.85 g/L) of C. reinhardtii in co-culture increased 10.3%, 51.3% and 55.7% respectively. The specific lipid synthetic rate (0.68 mg/(g·d)), lipid productivity (1.95 mg/(L·d)) and lipid content (220.4 mg/g) increased 33.3%, 107.5% and 32.0% respectively. Also, the proportions of saturated fatty acids and monounsaturated fatty acids C18-1 and C18-2 in the lipid elevated, indicating the suitability for biofuel production. [Conclusion] Simplicillium lanosoniveum promotes cell growth and lipid synthesis of C. reinhardtii, and thus algae-fungi co-cultivation can be considered in biofuel feedstock production.