细菌L-天冬氨酸α-脱羧酶的分子机制及分子改造研究进展

Advances in molecular mechanism and modification of bacterial L-aspartate alpha-decarboxylase

摘要:

L-天冬氨酸α-脱羧酶能够催化L-天冬氨酸生成β-丙氨酸，是泛酸代谢中的关键酶之一，对生物体中的能量代谢和脂肪代谢至关重要。细菌L-天冬氨酸α-脱羧酶属于丙酮酰依赖型的一类酶，具有特别的翻译后加工机制和底物失活作用，本文对L-天冬氨酸α-脱羧酶的分子机制和分子改造进行了综述，并对其在β-丙氨酸合成方面的应用进行了展望。

Abstract:

L-aspartate alpha-decarboxylase could catalyze the decarboxylation of L-aspartic acid to generate beta-alanine, which is one of key enzymes in pantothenate metabolism, playing an important role in energy and lipid metabolism of all lives. It belongs to a small class of pyruvoyl-dependent enzymes, which has characteristic mechanisms of self-cleavage on pro-enzyme and substrate inactivation during catalysis. Here, we reviewed advances and achievements in molecular mechanism and modification of bacterial L-aspartate alpha-decarboxylase, and expected its application in biosynthesis of beta-alanine.