禽源多重耐药金黄色葡萄球菌耐药基因检测及分子分型

Resistance genetic testing and molecular typing of multidrug-resistant Staphylococcus aureus in poultry

摘要:

【背景】金黄色葡萄球菌是革兰氏阳性菌，在动物和人身上能引起一系列疾病。【目的】了解安徽省不同地区禽源多重耐药金黄色葡萄球菌耐药性的情况及基因分型特征。【方法】以安徽不同地区的病禽肝脏作为标本，分离鉴定得到103株多重耐药金黄色葡萄球菌，并进行耐药基因型检测和ERIC-PCR分子分型。【结果】耐药菌株从三重到八重耐药均有分布，主要集中在五重(43/103)、四重(21/103)和六重耐药(22/103)。药敏结果显示，β-内酰胺类的耐药率最高(79.6%)，氨基糖苷类次之(71.8%)。耐药基因检出率由高到低分别为mecA (92.2%)、aac(6′)/aph(2″) (76.7%)、ermC (37.9%)、ermA (13.6%)和femA (3.9%)。ERIC-PCR分子分型获得6种不同类群，优势流行菌群为类群Ⅱ(38/103)。【结论】安徽地区金黄色葡萄球菌存在较严重的耐药性，氨基糖苷类、β-内酰胺类和大环内酯类抗生素的耐药基因携带率较高。分型结果表明安徽部分区域耐药金黄色葡萄球菌具有遗传多样性，但耐药谱与ERIC-PCR分子分型无明显关联。

Abstract:

[Background] Staphylococcus aureus is a Gram-positive bacterium that can become a pathogen causing an array of diseases in animals and people. [Objective] To understand the multiple drug resistant situation of poultry Staphylococcus aureus in different areas of Anhui Province, and its characteristics of drug resistance and genotyping. [Methods] Sick poultry livers collected from different areas of Anhui Province as inoculation materials. In total, 103 multiple drug-resistant strains of Staphylococcus aureus were isolated. Then the resistant genotypes and molecular typing of Staphylococcus aureus were detected by PCR and ERIC-PCR respectively. [Results] The strains were resistant to 3 to 8 antibiotics. Most strains demonstrated multi-drug resistance to 5 antibiotics (43/103), followed by 4 antibiotics (21/103) and 6 antibiotics (22/103). The drug resistance rate of Staphylococcus aureu to β-lactam was the highest (79.6%), followed by aminoglycosides (71.8%). The detection rate of drug resistance gene from high to low were mecA (92.2%), aac(6′)/aph(2″) (76.7%), ermC (37.9%), ermA (13.6%) and femA (3.9%). Six different groups were obtained by ERIC-PCR molecular typing, and the dominant epidemic flora was the group Ⅱ (38/103). [Conclusion] Drug resistant genes of aminoglycosides, β-lactam and macrolides had high detection rates. There were genetic diversities among avian Staphylococcus aureus in areas of Anhui Province according to molecular typing. There was no significant correlation between ERIC-PCR molecular typing and drug resistance spectrum.