响应面法优化粉被虫草中虫草素的提取工艺

Optimization of extraction of cordycepin from Cordyceps pruinosa by response surface methodology

摘要:

【背景】粉被虫草是民间常用药用真菌，具有抗辐射等多种药理作用，虫草素是其重要的活性物质。【目的】探究粉被虫草中提取虫草素的影响因素，为制取虫草素提供参考。【方法】以虫草素得率为指标，用响应面法优化超声提取工艺。分别考察超声时间、液料比和超声功率对提取效率的影响，在单因素实验基础上进行3因素3水平的响应面分析实验。【结果】最优提取条件为超声时间606 s、液料比45.9:1、超声功率400 W，在此条件下虫草素得率为6.136 mg/g，与模型预测值接近，方程拟合良好。【结论】首次对粉被虫草中虫草素的提取条件进行了研究，将对制取虫草素提供参考，有利于粉被虫草的深度开发利用。

Abstract:

[Background] Cordyceps pruinosa is a traditional medicinal fungus, which has many kinds of pharmacological effects such as anti-radiation. Cordycepin is an important bioactive compound of this fungus. [Objective] The influencing factors of cordycepin extracted from Cordyceps pruinosa were studied, so as to provide reference for the preparation of cordycepin from the fungus. [Methods] With extraction yield of cordycepin as the target, the response surface methodology was used to optimize the ultrasonic extraction process. The effects of ultrasonic time, liquid-to-solid ratio and ultrasound power on the extraction efficiency were studied using single factor experiment, then a three levels of response surface methodology experiments was chose to optimize the extraction process. [Results] The results showed that the optimum extraction conditions were: ultrasonic time 606 s, liquid-to-solid ratio 45.9:1, ultrasound power 400 W. A cordycepin yield of 6.136 mg/g was obtained under this condition, which was close to the model predicted value and showed the fitting equation was good. [Conclusion] In this experiment, the extraction conditions of cordycepin in Cordyceps pruinosa were studied for the first time, which would provide reference for the production of cordycepin from Cordyceps pruinosa and be helpful for deep use of this fungus.