纳帕海高原湿地浮游病毒丰度及其与可溶性有机碳关系

Virioplankton abundance and its relationship with dissolved organic carbon in Napahai plateau wetland

摘要:

【背景】浮游病毒在有机碳循环中具有重要作用。【目的】研究纳帕海高原湿地不同季节水样及土样中的浮游病毒和细菌丰度，并分析不同季节浮游病毒丰度与可溶性有机碳的关系。【方法】利用荧光显微镜技术检测不同季节水样中浮游病毒和细菌丰度，利用流式细胞仪技术检测不同季节土壤样品中病毒颗粒和细菌丰度。【结果】雨季所有样品浮游细菌和浮游病毒丰度分别为3.38×106/mL和4.38×107/mL，旱季所有样品的浮游细菌和浮游病毒丰度分别为8.85×105/mL和9.66×106/mL，浮游细菌和浮游病毒丰度年平均值分别为2.13×106/mL和2.67×107/mL。不同季节浮游细菌和浮游病毒的丰度有显著性差异，雨季明显高于旱季(P<0.01)。雨季细菌碳产量为8.01 μg C/(L·h)，旱季为10.30 μg C/(L·h)。雨季浮游病毒裂解细菌贡献的可溶性有机碳(Dissolved organic carbon，DOC)占总DOC库的32.38%?76.38%，而旱季为8.23%?47.87%。【结论】浮游病毒在纳帕海高原湿地有机碳循环中具有重要作用。

Abstract:

[Background] Virioplankton plays an important role in organic carbon cycle. [Objective] We studied the abundance of virioplankton and bacteria in different seasonal water samples and soil samples in Napahai wetland, and analyzed the relationship between the abundance of virioplankton and soluble organic carbon in different seasons. [Methods] The abundance of virioplankton and bacteria in water samples of different seasons was detected by Epifluorescence Microscopy. The abundance of virus and bacteria in soil samples of different seasons was detected by Flowcytometry. [Results] The abundance of phytoplankton bacteria and virus was 3.38×106/mL and 4.38×107/mL in rainy season. The abundance of phytoplankton bacteria and virus in the dry season was 8.85×105/mL and 9.66×106/mL, the average annual abundance of phytoplankton bacteria and virus was 2.13×106/mL and 2.67×107/mL. There were significant differences of phytoplankton bacteria and virus abundance in different seasons. The yield of bacterial carbon was 8.01 μg C/(L·h) in the rainy season, and 10.30 μg C/(L·h) in the dry season. The DOC contributed by the virioplankton accounted for 32.38% to 76.38% of the total DOC library in the rainy season, while it was 8.23% to 47.87% in the dry season. [Conclusion] Virioplankton plays an important role in the organic carbon cycle of the Napahai wetland.