热带普通小球藻培养模式的筛选及其培养基的优化

Optimization of tropical Chlorella vulgaris culture method and its medium formulation

摘要:

【背景】从海南热带海区中分离得到一株微藻，其生长速度快、适应力强，经鉴定该微藻为普通小球藻。【目的】提高热带普通小球藻的生长速率。【方法】以“宁波大学3#微藻培养液配方”为基础培养液，分别添加有机碳(C6H12O6和CH3COONa)对热带普通小球藻进行自养、兼养及异养培养，获得促进热带普通小球藻快速生长的培养方式。在“宁波大学3#微藻培养液配方”的基础上对热带普通小球藻的兼养培养基配方进行优化，并用优化兼养培养基与“宁波大学3#微藻培养基”对比培养热带普通小球藻。【结果】添加6 g/L CH3COONa的兼养模式促进热带普通小球藻生长效果最好；优化的兼养培养基配方为：6 g/L CH3COONa，20 mg/L (NH4)2SO4-N，5 mg/L NaH2PO4-P，3 mg/L FeSO4-Fe，1 mg/L Vitamin B1和0.000 5 mg/L Vitamin B12。对比培养实验结果显示，培养第6天，兼养培养液收获的生物量(细胞密度)达4.20×107 cells/mL，是“宁波大学3#配方微藻培养液”的2.30倍。【结论】兼养培养模式为热带普通小球藻的最佳培养模式，优化的兼养培养基极显著地提高了热带普通小球藻的生物量(P<0.01)。

Abstract:

[Background] A strain of microalgae was isolated from the tropical sea of Hainan province, and it has a high growth rate and strong adaptability. The microalga was identified as Chlorella vulgaris. [Objective] To improve the growth rate of C. vulgaris. [Methods] Based on the formulation of “Ningbo University 3#” medium, organic carbon of C6H12O6 and CH3COONa were added for the autotrophic culture, mixed culture and heterotrophic culture, respectively, and also, the mixotrophy medium of C. vulgaris was further optimized. [Results] The mixed culture method with 6 g/L CH3COONa showed the highest growth rate of C. vulgaris, comparing with heterotrophic and autotrophic culture method. The optimal formulation of mixotrophy medium was as followed as 6 g/L CH3COONa, 20 mg/L (NH4)2SO4-N, 5 mg/L NaH2PO4-P, 3 mg/L FeSO4-Fe, 1 mg/L Vitamin B1 and 0.000 5 mg/L Vitamin B12. The biomass (cell density) of the “mixotrophy medium” was up to 4.20×107 cells/mL by the end of the sixth day, being 2.30 times higher than the cell density of “Ningbo University 3#” medium. [Conclusion] The mixotrophic mode is optimal to the cultivation of C. vulgaris as compared with other two cultivation modes, and the optimized mixotrophic medium significantly enhanced the biomass (P<0.01).