16S rRNA基因高通量测序法分析黄河太岁细菌的多样性

Bacterial diversity of the Yellow River Taisui by 16S rRNA gene sequencing

摘要:

【背景】太岁在我国的记载由来已久，《神农本草经》记载其具有扶正固本、轻身不老的功效。但是，太岁作为一种生物体，它的组成分类尚不明确，因而其药用价值得不到有效的科学验证。因此，利用各种生物技术和手段来客观地分析太岁的成分，为利用和开发太岁提供科学依据。【目的】检测太岁(编号D15112285)中所含细菌的种类，探究太岁中可能存在的原核微生物的种类及其之间的关系。【方法】采用Illumina MiSeq 2×250系统对黄河太岁的细菌16S rRNA基因(V4区)进行研究，利用FLASH等软件对数据进行分析。【结果】共获得OTU (operational taxonomical unit) 626条，涉及19门49纲80目107科112属。在属的水平上前十的优势菌群有Bacteroides、Coprococcus、Escherichia、Ruminococcus、Lactobacillus、[Ruminococcus]、Oscillospira、Faecalibacterium、Shewanella和Halomonas。【结论】黄河太岁中存在多种不同种类的细菌。

Abstract:

[Background] Taisui has a history of use in China dating to ancient times. In Shennong Bencaojing, it is listed as a medicine capable of supporting healthy qi, strengthening the body’s constitution and promoting longevity. However, as an organism, its constituents and classification are still unclear causing inadequate identification of its medicinal value. It is highly necessary to analyze its components objectively using modern biological analytical techniques. [Objective] To determine the existence of prokaryotes in Taisui, and to analyze the types of bacteria and the relationships among them. [Methods] The Illumina MiSeq 2×250 platform was used to carry out sequencing of V4 regions of the 16S rRNA gene of bacteria in the Yellow River Taisui. FLASH and other software packages were used to analyze the data. [Results] A total of 626 operational taxonomical units were found, involving 19 phyla, 49 classes, 80 orders, 107 families, and 112 genera. The top 10 dominant bacteria at the genus level were found to be Bacteroides, Coprococcus, Escherichia, Ruminococcus, Lactobacillus, [Ruminococcus], Oscillospira, Faecalibacterium, Shewanella, and Halomonas. [Conclusion] There are different microbial communities of bacteria in the Yellow River Taisui.