Abstract:Application of “Internet +” technology in education area makes great changes in teaching scene, the role of teacher and the characteristics of undergraduate students’ learning styles. In order to adapt to the teaching characteristics under “Internet +” era and the needs of students’ personalized learning and cultivating innovative ability, smart teaching tools were introduced into the teaching of Microbiology Experiment course and the online open course, which was constructed on the internet learning platform. The teaching tool——RainClassroom has the function of transforming intelligent mobile phone into students’ learning tool. Application of this learning tool enhances the undergraduate students’ learning enthusiasm, strengthens the interaction between the teacher and students, and achieves the timely feedback of teaching results; the students’ learning data collected through this tool is valuable for teachers to evaluate the learning results objectively and to optimize the personalized teaching. Furthermore, the online opening course Module Microbiology Experiment was constructed on the platform of Jiangsu online course center. Under the process of curriculum construction, teachers should update the teaching concept in time and improve the enthusiasm of participating in curriculum construction; the curriculum constructors should receive the professional training for online open courses construction; the production and selection of course contents should consider the participants’ characteristics, the costs of the teaching video production, and the learners’ studying curve. The construction of online opening course will provide a fundamental support upon which flipped classroom teaching and blended teaching can be applied for the reform of Microbiology Experiment course under “Internet +” era.