Curiosity is the driving force of research and learning. Our aim is how to stimulate and continue students’ curiosity in Microbiology Experiment courses to improve teaching effectiveness. We attempt to combine “Detection of environmental microbes” with microbiological identification to comprehensive completion of the major Microbiology experiments. The curiosity, enthusiasm and interest excited from “Detection of environmental microbes” experiment were throughout the microbiological experiments by using microbiological identification. Consequently, the students were promoted to practice actively, initiatively and diligently in every experiment, and to deep understand the experimental knowledge and mastery of experimental skills. This combination between experiment teaching and extracurricular research contributes to cultivate students’ basic scientific research ability and innovative spirit, and to obtain the better teaching effect.