Abstract:Environmental Engineering Microbiology is a core course for environmental majors, which plays an important role in the forming of students’ knowledge structure and in the development of students’ application skills. In order to realize the target of the course and achieve the learning expectation of it, the concept of Outcomes-based education (OBE) is employed in the teaching reform, and the reverse-design and positive-implementation principle is also put forward in the teaching of Environmental Engineering Microbiology. The reverse-design and positive-implementation principle is to optimize the teaching process according to the expected course target, which includes the choice of teaching content, the enhancement of the coordination of Environmental Engineering Microbiology and other courses, and the strengthening of students’ dominant role in the teaching implementation. Through the reform of teaching methods, we can promote the expected learning output and construct a reasonable learning output evaluation system. Although the OBE concept has been introduced into the teaching implementation, and some achievements have been made, the construction of the system has yet to be perfected.