Abstract:The Microbiology Digital Curriculum is a curriculum that based on the content of Microbiology knowledge and is disseminated through ICC platform of the Higher Education Press. The Microbiology Digital Curriculum compiled by authors has been published by the Higher Education Press, and this paper introduces the design and teaching applications of it. The structural design includes chapter titles, assignments, basic questions, group discussion, course papers and free discussion Forum; Resource design includes teaching videos, digital resources (teaching courseware, animation, etc.), and online self-testing; Interactive design includes discussion, reviews, troubleshooting and so on. This paper also introduces how to build an online teaching mode: teacher publishing curriculum resources and learning tasks before classes on the platform—students logging on to watch resources—teachers answering questions and summarizing knowledge in classes—students discussing questions and making reports—after-class students submitting homework and self-tests. The results of teaching practice show that the Microbiology Digital Curriculum plays a positive role in improving students’ interests, cooperative learning ability, information technology accomplishment and learning ability.