未培养微生物分离培养技术研究进展

Advance in isolation and culture techniques of uncultured microbes: a review

摘要:

自然环境中的微生物具有多样性高的特点，而99%的微生物使用传统的方法仍然是“不可培养的”，并且能被培养的小部分微生物不能够代表微生态的多样性。因此，确定新的微生物物种和功能仍然是目前的重要任务。本文将综述基于调整培养基、考虑微生物间相互作用、捕获分离培养、高通量分离培养来分离培养微生物群体中的未培养微生物的方法，及功能性研究的进展情况。

关键词:未培养微生物，分离培养，技术方法

Abstract:

Microbes have the characteristic of high diversity in natural environment, but 99% of microorganisms remain “unculturable” by using traditional methods. Besides, the culturable fraction can’t represent the diversity of micro-ecology. Therefore, the present tasks are to identify species and functions of new microorganisms. This article reviews some success in the isolation and cultivation of unculturable bacteria from complex bacterial communities and their functions researches, including modifying medium, microbial interactions, capture, isolation and cultivation and high-throughput cultivation.

Key words:Uncultured microbes, Isolation and culture, Techniques