内生菌株B16发酵条件优化及其对人参锈腐病的防效

Optimization of B16 endophytic strain culture for its control of ginseng Cylindrocarpon destructans

摘要:

【目的】优化人参病害拮抗菌株B16的发酵条件，提高发酵液的活菌含量和抗菌活性，检测该菌对人参病害的防效。【方法】采用单因子试验、正交试验优化菌株B16的发酵培养基及发酵条件，于室内盆栽条件下研究其对人参锈腐病的防效。【结果】菌株B16发酵最适培养基为：蔗糖1.00%、酵母膏0.50%、MgSO4·7H2O 0.05%、FeSO4·7H2O 0.06%、NaCl 1.00%；最佳发酵条件：pH 7.5、温度35 °C、接种量5%、装液量40 mL/250 mL、摇床转速170 r/min、发酵周期48 h。菌株B16发酵液对人参锈腐病的保护作用和治疗作用防效分别达到64.8%和58.6%。【结论】菌株B16具有很强的生防潜力。

Abstract:

[Objective] To study the optimal fermentation conditions of antagonistic strain B16 isolated from ginseng root for improving the quantity and antibacterial activity of the bacteria, and test its control effect on ginseng disease. [Methods] The single factor test and orthogonal test were used in optimizing the fermentation media and the conditions, and the strain’s antagonistic effects to ginseng pathogenic fungus Cylindrocarpon destructans were tested with pot trails. [Results] Optimum fermentation media for B16 was: sucrose 1.00%, yeast extract 0.50%, MgSO4·7H2O 0.05%, FeSO4·7H2O 0.06% and NaCl 1.00%; and the optimum conditions are: pH 7.5, 35 °C, 5% 108?CFU/mL bacterium suspension, 40 mL/250 mL, 170 r/min and 48 h fermentation. The B16 fermentation broth shows antagonistic effect to ginseng pathogenic fungus C. destructans, the protection effect and curation effect are 64.8% and 58.6%, respectively. [Conclusion] Strain B16 has potential for C. destructans biocotrol.