[Objective] To understand the complete genome sequence information of Mycoplasma mycoides subsp. capri str. PG3 (Mmc str. PG3) and offer help for screening of main antigenic genes. [Methods] The high flux Illumina HiSeq 2000 technology was used for genome sequencing and annotation of Mmc str. PG3. [Results] The genome of Mmc str. PG3 consisted of a circular chromosome of 1 025 065 bp with an overall molar G+C% content of only 23.6%. The chromosome of Mmc str. PG3 contained 846 predicted coding sequences. The majority of genes were associated with protein translation, ribosome synthesis and structure, DNA replication and repair, glycometabolism and environment transfer and conversion processes. The Mmc-specific maltodextrin/maltose gene cluster was also identified in the genome of Mmc str. PG3. Comparative genomic analysis showed that Mmc str. PG3 should have significant genomic synteny with Mmc str. 95010. Three genes (GL000459, GL000461, GL000462) were identified as variable lipoprotein genes in the genome of Mmc str. PG3 by genomic information analysis. [Conclusion] Complete genome sequence of Mmc str. PG3 provided in this study might facilitate genome-wide structural annotation to assess genes associated with pathogen virulence. The results of this study would provide a theoretical basis for further research on the role of variable surface lipoprotein of the pathogenesis of mycoplasmose and development of biological engineering vaccine.
