假单胞菌H78中全局调控蛋白Crc对Plt生物合成及其基因表达的调控

Regulation of pyoluteorin (Plt) biosynthesis and gene expression by global control protein Crc in Pseudomonas protegens H78

摘要:

【目的】研究Pseudomonas protegens H78中全局调控蛋白Crc对藤黄绿菌素(Pyoluteorin，Plt)生物合成及其基因表达的调控。【方法】通过同源重组方法无痕敲除crc基因，并将H78Δcrc突变株与H78野生株在KMB培养基中发酵测定Plt产量；采用lacZ报告分析研究Crc对plt合成基因表达的调控。【结果】突变株H78Δcrc的Plt产量显著下降；Crc在整体水平、转录水平及转录后水平均正调控plt合成基因的表达。【结论】全局调控因子Crc对Plt合成及基因表达表现为正调控作用。

Abstract:

[Objective] The aim of this study was to study the influence of Crc (Cataboliterepression control protein) on pyoluteorin (Plt) biosynthesis and gene expression in Pseudomonas protegens H78. [Methods] The crc gene in the H78 chromosome was knocked out by homologous recombination. Plt production of H78 strain and its mutant grown in KMB was measured by HPLC. The regulation of Crc on plt operon expression was assessed by the lacZ reporter analysis. [Results] Plt production was significantly reduced in H78Δcrc. Crc positively regulated the expression of plt biosynthetic operon at the overall level, the transcriptional level and the post-transcriptional level. [Conclusion] The global regulator Crc positively regulates Plt production and its operon expression.