传染性支气管炎病毒N蛋白CTL表位与BF2*15鸡MHC I基序的相互作用

Interactions of the major histocompatibility complex class I BF2*15 and cytotoxic T lymphocyte epitopes from nucleoprotein of avian infectious bronchitis virus

摘要:

【目的】近年来鸡传染性支气管炎病毒在国内鸡群呈现流行趋势，尤其是变异毒株的出现，加剧了对鸡群的危害。鸡主要组织相容复合体蛋白(MHC I)通过特定的基序结合抗原表位多肽，进而识别感染病毒的细胞并引起免疫反应，达到清除病毒的效果。鉴定BF2*15鸡主要组织相容复合体(MHC I)的结合基序。【方法】采用同源建模、分子动力学和分子对接等计算方法，构建了传染性支气管炎病毒(IBV) N蛋白表位多肽和鸡MHC I BF2*15之间的复合物结构，来探索BF2*15识别抗原表位多肽的潜在结合基序。【结果】通过对该复合物的相互作用关系分析，鉴定出BF2*15鸡主要组织相容复合体(MHC I)的一条潜在结合基序“x-Arg-x- x-x-Arg”。【结论】解释了传染性支气管炎病毒N蛋白CTL表位与BF2*15鸡MHC I基序的相互作用原理，该研究结果对了解传染性支气管炎病毒免疫机制以及基于特定基序筛选和设计通用疫苗具有借鉴意义。

Abstract:

[Objective] In recent years, the epidemic of infectious bronchitis virus in China has become a popular trend in domestic flocks, especially the emergence of mutant strains, which aggravate the harm to the flocks. Major histocompatibility complex (MHC I) class I is able to bind antigens containing certain motif and further recognize and eradicate cells affected by virus. Identification of the binding motif of the complex of chicken MHC I protein BF2*15. [Methods] The complex of chicken MHC I protein BF2*15 and Nucleoprotein of infectious bronchitis virus (IBV) octapeptide were constructed by computational method, including homology modeling, molecular dynamics simulation and molecular docking. [Results] According to the interaction relationship of BF2*15 and IBV Nucleoprotein peptide, we conclude that “x-Arg-x-x-x-Arg” may represent a potential binding motif of BF2*15. [Conclusion] Interactions of the major histocompatibility complex class I BF2*15 and cytotoxic T lymphocyte epitopes from nucleoprotein of avian infectious bronchitis virus was illuminated in this study, and the results may shed light on the understanding of immune mechanism of IBV and research towards universal IBV vaccine.