Trichosporon montevideense WIN合成纳米金的催化特性

Catalytic characteristics of gold nanoparticles biosynthesized by Trichosporon montevideense WIN

摘要:

【目的】考察菌株Trichosporon montevideense WIN合成纳米金的催化特性及应用。【方法】利用活性WIN菌作用不同浓度HAuCl4 (1、2和4 mmol/L)合成纳米金的特性，分别利用活性WIN菌和灭活WIN菌合成纳米金，分析合成纳米金的形貌、粒径及其催化特性。【结果】HAuCl4浓度为1 mmol/L时，菌株WIN合成了纳米金，HAuCl4浓度为2 mmol/L和4 mmol/L时，菌株WIN合成了纳米金及较大尺寸的金颗粒。通过紫外-可见光谱扫描、透射电子显微镜分析，发现活性和灭活WIN菌均能还原Au3+合成纳米金，合成的纳米金均以球形为主，还有少量三角形、四边形及六边形。活性WIN菌合成的纳米金粒径范围为3 nm?252 nm，平均粒径为45.2 nm，而灭活WIN菌合成的纳米金为1 nm?271 nm，平均粒径为38.3 nm。活性和灭活WIN菌合成的纳米金对还原4-硝基苯酚的催化速率分别为2.76×10?3 s?1和4.84×10?3 s?1。【结论】菌株Trichosporon montevideense WIN的活性及灭活细胞均可以合成纳米金，且合成的纳米金具有良好的催化特性，在催化去除环境中难降解污染物中具有一定的应用前景。

Abstract:

[Objective] To examine the catalytic characteristics of gold nanoparticles (AuNPs) biosynthesized by Trichosporon montevideense WIN. [Methods] The characteristics of gold particles synthesized by incubation strain WIN with 1 mmol/L, 2 mmol/L and 4 mmol/L HAuCl4 were investigated. In addition, the AuNPs were biosynthesized by active and inactive cells of strain WIN, respectively. The morphology and particle size of the synthesized AuNPs were analyzed, and the catalytic activity of AuNPs for reduction of nitro aromatic compounds was also evaluated. [Results] When the concentration of HAuCl4 was 1 mmol/L, AuNPs were produced by strain WIN, while AuNPs and large gold particles were formed with 2 mmol/L and 4 mmol/L HAuCl4. The active and inactive cells of strain WIN could synthesize AuNPs based on the analyses of UV-vis spectroscopy and transmission electron microscopy. The synthesized AuNPs were mainly spherical, and some triangle, hexagon and parallelogram. The size of AuNPs synthesized by active cells of strain WIN ranged in 3 nm?252 nm with an average size of 45.2 nm, while the size range and average size of AuNPs synthesized by inactive cells of strain WIN were 1 nm?271 nm and 38.3 nm, respectively. The AuNPs synthesized by active and inactive cells of strain WIN could effectively catalyze the 4-nitrophenol reduction with the catalytic rate constants of 2.76×10?3 s?1 and 4.84×10?3 s?1, respectively. [Conclusion] Trichosporon montevideense WIN could be used to synthesize AuNPs and the biosynthesized AuNPs exhibited good catalytic properties, which showed a potential application in catalytic removal of the refractory pollutants in environment.