外源化学物质对副溶血性弧菌药物敏感性的影响

Effect of additional chemicals on antimicrobial susceptibility of Vibrio parahaemolyticus

摘要:

【目的】细菌对抗生素的耐药性已成为全球公共卫生问题关注的热点。有研究表明外源添加化学物质可以增强耐药细菌对抗生素的敏感性。本研究比较了3种化学物质葡萄糖、丙氨酸、甘油对增强副溶血性弧菌抗生素敏感性的作用效果。【方法】在亚抑菌浓度抗生素胁迫条件下，通过比较副溶血性弧菌在添加终浓度为10 mmol/L葡萄糖、丙氨酸、甘油后细菌存活率随时间的变化水平，来观察弧菌对亚抑菌浓度抗生素敏感性作用效果的改变，并采用氧化磷酸化解偶联剂CCCP对实验结果进行验证。【结果】发现3种外源化学物质均能增强亚抑菌浓度氨基糖苷类抗生素对副溶血性弧菌的杀菌能力，其中外源添加葡萄糖对增强亚抑菌浓度卡那霉素的杀菌能力最为显著，而对其他种类抗生素的杀菌能力则无明显增强作用。加入氧化磷酸化解偶联剂CCCP后可消除由外源化学物质引发的弧菌抗生素敏感性作用增强的现象。【结论】通过调节细菌细胞代谢水平可提高耐药副溶血性弧菌对氨基糖苷类抗生素的敏感性，对多重耐药副溶血性弧菌的防控具有一定的实际应用价值。

Abstract:

[Objective] Antibiotic resistance is a severe public health problem. Recently, the relationship between bacterial metabolic regulation and antibiotic efficacy has been proposed in several pathogens. In the present study, we explored the effect of adding additional chemicals on drug susceptibility of Vibrio parahaemolyticus. [Methods] We studied the phenotypic change and compared the survival of V. parahemolyticus after treatment with antibiotics and different chemicals. The experimental results were conformed with carbonylcyanide-p-chlorophenyl hydrazine (CCCP). [Results] These results indicated that specific chemicals effectively potentiate kanamycin to eliminate multidrug resistant V. parahemolyticus. Among them, the addition of exogenous glucose could significantly enhance the sterilization ability with subinhibitory concentrations of kanamycin, but without effect on other antibiotics. The enhancement of antimicrobial susceptibility of Vibrio parahaemolyticus caused by exogenous chemicals could be eliminated after treatment with CCCP. [Conclusion] The sensitivity of V. parahemolyticus to aminoglycosides can be improved by regulating the metabolism of bacterial cells. Therefore, we present a novel approach in fighting against multidrug resistant V. parahemolyticus with practical application value.