Abstract:Micro-lecture is a kind of visible learning resource which is generated for online learners and designed with learner centered. The application of micro-lecture often combined tightly with flipped classroom. In order to solve problems associated with the teaching and learning of the course Food Microbiology Experiment, an experimental teaching mode of flipped classroom based on micro-lecture video was developed, which included five aspects of “video, platform, watching, imitation and evaluation”. The teaching mode has shifted the course from “teaching predominant” to “learning predominant”, as well as from “knowledge transfer predominant” to “ability training predominant”. As a result, the strategy improved students’ autonomous learning ability and learning interest, hence it obviously improved the teaching effectiveness.