新疆伊犁地区原牛乳中乳酸菌的多样性分析

Biodiversity of lactic acid bacteria isolated from raw milk of Yili area in Xinjiang

摘要:

【目的】对新疆伊犁地区原牛奶中乳酸细菌的遗传多样性进行分析。【方法】采用菌落培养、rep-PCR (repetitive genomic fingerprinting)指纹图谱和16S rRNA基因序列分析相结合的方法研究牛乳内乳酸菌的遗传多样性。【结果】从5份原牛乳中分离出乳酸菌29株，基因序列分析和系统进化分析显示29株乳酸菌隶属于5个属，分别为：Lactococcus、Lactobacillus、Leuconostoc、Pediococcus和Enterococcus。优势属为Leuconostoc (27.6%)，其次为Lactococcus (24.0%)。【结论】新疆伊犁地区原牛乳中乳酸菌多样性丰富，为开发新疆地区益生乳酸菌提供了丰富的活性资源。

Abstract:

[Objective] The genetic diversity of lactic acid bacteria which were isolated from raw milk of Yili area in Xinjiang was analyzed. [Methods] The diversity of lactic acid bacteria was analyzed by using pure culture, repetitive genomic fingerprinting (rep-PCR) analysis patterns and 16S rRNA gene sequence analysis. [Results] A total of 29 strains of lactic acid bacteria were obtained from five raw milk samples. The phylogenetic analysis showed that these strains belonged to five phylogenetic groups, they were Lactococcus, Lactobacillus, Leuconostoc, Pediococcus and Enterococcus respectively. The dominant genus was Leuconostoc (27.6%), followed by Lactococcus (24.0%). [Conclusion] The abundant diversity of lactic acid bacteria in the raw milk of Yili area in Xinjiang provided rich resources for exploiting probiotic lactic acid bacteria products.