大熊猫肠道放线菌的种群组成及多样性分析

Diversity and community structure of gut actinobacteria of giant panda

摘要:

【目的】探究不同年龄、不同性别大熊猫肠道放线菌的多样性及群落结构，为寻找潜在产生活性化合物的放线菌资源提供理论依据。【方法】采用PCR-DGGE技术对大熊猫肠道放线菌进行分析，对电泳结果进行UPGMA聚类分析、主成分分析、生物多样性等多重比较。【结果】变性梯度凝胶电泳(DGGE)图谱显示，不同大熊猫肠道中放线菌的多样性及群落结构存在明显差异。随着年龄的增长，雌性大熊猫肠道中放线菌的多样性指数(H')和丰富度(S)逐渐减少，而雄性大熊猫肠道内放线菌的多样性指数(H')和丰富度(S)逐渐增多。不同个体的大熊猫肠道放线菌的群落结构存在明显差异，但相同性别之间的相似性很高。DGGE条带回收测序结果表明，获得的28条序列归属于10个放线菌属，其中链霉菌属(Streptomyces)为优势菌属，占总数的46%；北里孢菌属(Kitasatospora)、红球菌属(Rhodococcus)、棒杆菌属(Corynebacterium)、迪茨氏菌属(Dietziaceae)、大理石雕菌属(Marmoricola)、布登堡菌属(Beutenbergia)、微杆菌属(Microbacterium)、链嗜酸菌属(Streptacidiphilus)和芽生球菌属(Blastococcus)等为非链霉菌属，占总数的54%。【结论】大熊猫肠道内蕴藏着丰富的放线菌资源，其肠道菌群的结构与组成受年龄和性别的影响。

Abstract:

[Objective] Study the diversity and community structure of gut actinobacteria of giant panda with the different age and gender, in order to provide scientific basis for searching the actinobacteria possessing potential to produce bioactive compounds. [Methods] Denaturing gradient gel electrophoresis (DGGE) was employed to reveal the gut actinobacteria diversity and community structure of giant panda, and the results were analyzed by multiple comparisons, including UPGMA cluster, PCA and biological diversity of gut actinobacteria in different age and gender. [Results] The diversity and community structure of gut actinobacteria in different panda exhibited significant differences by DGGE analysis. The Shannon-Wiener index (H') and Richness (S) of gut actinobacteria in female giant pandas decreased as they grew older, but the male giant pandas were opposite. The different diversity of gut actinobacteria were observed in different giant panda. However, the diversity index of gut actinobacteria from the same gender possessed higher similarity. The recovery of DGGE strip series showed the 16S rRNA gene sequence similarity between the 28 stronger bands and the typical strains published in GenBank was 96% to 100%, and those sequences belonged to ten genera, Streptomyces was dominant genera accounting for 46% of all sequences, the remainder belonged to genera Kitasatospora, Rhodococcus, Corynebacterium, Dietziaceae, Marmoricola, Beutenbergia, Microbacterium, Streptacidiphilus, Blastococcus, which account for 54%. [Conclusion] This result revealed the intestinal actinobacteria community structure and diversity of giant panda were different and abundant, which were influenced by their age and gender.