废烟叶提取液源尼古丁降解菌分离鉴定和特性

Isolation, identification and characteristics of nicotine degrading strain from tobacco waste extract

摘要:

【目的】目前造纸法再造烟叶工艺已经成为我国重要的废烟叶处理和利用方式，该工艺中尼古丁的调控是很重要的待解决问题。从废烟叶提取液(Tobacco waste extract，TWE)中筛选高耐受高活性降解尼古丁微生物用来直接处理烟梗或烟末提取液中的尼古丁，可实现对尼古丁指标的调控。【方法】以尼古丁为唯一碳氮源的基本培养基为筛选平板，从废烟叶提取液中筛选降解尼古丁菌株；对分离获得的菌株进行形态、生理生化和16S rRNA基因序列分析比对，鉴定其种属；获得的菌株分别在基本培养基和废烟叶提取液中考察其生长和尼古丁降解效果。【结果】从废烟叶提取液中获得了一株尼古丁降解活性和耐受力较好的降解菌株Pseudomonas sp. JY-Q，且在TWE中也有较强的降解能力。【结论】Pseudomonas sp. JY-Q可用于水体和TWE环境尼古丁的降解，但共存的葡萄糖对其有抑制作用，有待深入研究。

Abstract:

[Objective] Reconstituted tobacco technology has been one important method to treat and reuse the tobacco waste, in which nicotine control is a key problem to resolve. To realize the flexible control of nicotine, tobacco waste extract (TWE) was selected to screen high activity nicotine degrading strains for potential utilization in reconstituted tobacco process. [Methods] BSM containing nicotine as sole carbon and nitrogen source was used to screen nicotine degrading strains from TWE. Isolated strain identification includes morphology, physiology, and 16S rRNA gene sequence analysis. The activity of nicotine degradation of the isolate was evaluated in BSM and TWE respectively. [Results] The isolate Pseudomonas sp. JY-Q showed high degrading activity and tolerance of nicotine both in BSM and TWE. [Conclusion] Pseudomonas sp. JY-Q is a potential strain for nicotine degradation in aqueous environment and TWE. However, coexisting glucose inhibits its activity, which requires further research to resolve.