研究型模块实验“产淀粉酶菌株的筛选、发酵条件优化、菌种鉴定和选育”的教学实践

Teaching module for experimental practice on “screening of amylase-producing bacteria, optimization of fermentation conditions, bacterial identification, and strain improvement”

摘要:

采用模块化形式开展微生物学实验教学可提升学生的学习积极性，培养学生的探究性学习精神。“产淀粉酶菌株的筛选、发酵条件优化、菌种鉴定和选育”模块实验以产淀粉酶细菌为研究对象，安排学生开展培养基的配置、土壤样本采集和分离筛选产淀粉酶菌株、产淀粉酶菌株的发酵条件优化、菌种的形态学和分子生物学鉴定、紫外线诱变育种等5个单元实验。该模块实验以连贯性的研究任务引导学生进行探究性学习，将微生物实验操作技能的学习融合于探究性学习过程。实验以学生为主体，采用“固定+机动”方式安排教学任务，保证模块实验的整体性和连贯性。采用“模块论文+现场操作考试”的方式进行考核，提升学生的科研素养和强化学生对微生物基本操作技能的掌握。该研究型模块实验的教学提升了学生的微生物学研究热情，提高了微生物学课程群的教学质量。

Abstract:

The implementation of the experimental research module into microbiology experiment teaching improves undergraduate students’ motivation and engagement with inquiry-based learning. In this experimental research module “screening of amylase producing bacteria, optimization of fermentation conditions, bacterial identification, and strain improvement”, students will focus on studies of amylase-producing bacteria, and will be assigned with 5 experimental units that include preparation of culture medium, collection of soil samples and isolation of amylase-producing bacteria, optimization of fermentation conditions of the isolated amylase-producing bacterium, morphologic and molecular identification of bacterial strain, and strain improvement through ultraviolet mutagenesis. This experimental research module will guide students to experience an entire inquiry-based learning process by consistently planning and conducting research projects, through which students will learn and eventually master fundamental microbial experimental skills. This module is student-centered, implemented with “fixed plus mobile” mode, to ensure its integrity and consistency. To improve students’ scientific literacy and basic microbiological techniques, the examination is composed of two parts: scientific paper writing and onsite skills test. Overall, this experimental research module inspires students’ enthusiasm and motivation in learning microbiology; meanwhile it improves the teaching quality of microbiology curriculums.