虎耳草炭疽病病原菌鉴定

Identification of Colletotrichum species associated with Saxifraga stolonifera anthracnose

摘要:

【目的】从虎耳草炭疽病病斑分离病原菌，并对病原菌进行鉴定。【方法】采用柯赫氏法则对从病斑组织块分离的病原菌进行验证，通过形态学结合多基因分子系统学方法对病原菌进行鉴定。【结果】从虎耳草炭疽病病斑分离到2株菌株(菌株号：LPSU 20120244、LPSU 20120251)。2株菌株孢子均无色，无隔，直、圆柱状，菌株LPSU 20120244孢子(11?25) μm×(5?9) μm，菌株LPSU 20120251孢子(15?25) μm×(5?7) μm。多基因分子系统树中，2株菌株分别与喀斯特炭疽菌(Colletotrichum karstii Y.L. Yang，Zuo Y. Liu, K.D. Hyde & L. Cai)和江西炭疽菌(C. jiangxiense F. Liu & L. Cai)模式菌株聚为一支，支持率均为100%。【结论】形态学及多基因分子系统学分析表明菌株LPSU 20120244为喀斯特炭疽菌，菌株LPSU 20120251为江西炭疽菌。

Abstract:

[Objective] The study aims to isolate and identify the pathogen from Saxifraga stolonifera anthracnose. [Methods] The pathogen were isolated by tissue associated anthracnose and confirmed through Koch’s postulates, then the strains were identified through morphologic characteristics and multi-locus (ACT, CAL, CHS-1, GAPDH, ITS, TUB2) phylogeny analysis. [Results] Two strains, isolated from the leaves of S. stolonifera, were numbered as LPSU 20120244 and LPSU 20120251. Conidium of both two strains was hyaline, aseptate, straight, cylindrical. Conidium size of LPSU 20120244 was (11?25) μm×(5?9) μm, LPSU 20120251, (15?25) μm×(5?7) μm. Strain LPSU 20120244 and LPSU 20120251 was clustered with Colletotrichum karstii Y.L. Yang, Zuo Y. Liu, K.D. Hyde & L. Cai and C. jiangxiense F. Liu & L. Cai, respectively, and the bootstrap support of both two clades were 100%. [Conclusion] Based on morphologic characteristics and multi-locus phylogeny analysis, the two strains represent 2 Colletotrichum species, Colletotrichum karstii (strain LPSU 20120244) and C. jiangxiense (strain LPSU 20120251).