米曲霉M-4降解己烯雌酚的条件优化

Optimization of diethylstilbestrol degradation by Aspergillus oryzae M-4

摘要:

【目的】以米曲霉(Aspergillus oryzae) M-4对己烯雌酚(diethylstilbestrol，DES)的降解率为响应值，对其降解条件进行优化。【方法】采用Plackett-Burman法对培养基组分和降解条件筛选显著性影响因素，并通过Box-Bohnken设计试验优化降解条件。【结果】最优培养基配方为：蛋白胨1.3%，CaCl2 0.045%，葡萄糖0.5%，K2HPO4 0.15%，KH2PO4 0.05%，NaCl 0.05%，Tween 80 0.2%，DES质量浓度44 mg/L；最优培养条件为：初始pH 7.5，种龄72 h，转速140 r/min，培养温度28 °C，培养时间72 h。【结论】在最优条件下菌株M-4对DES降解率为83.89%，比优化前(60.98%)提高1.38倍，差异极显著(p<0.01)。

关键词:己烯雌酚，米曲霉，降解条件，优化

Abstract:

[Objective] We optimized degradation conditions for diethylstilbestrol (DES) by Aspergillus oryzae M-4. [Methods] Medium components and culture conditions were screened by using Plackett-Burman method. The degradation conditions were then optimized through Box-Bohnken design. [Results] The optimal medium was composed of 1.3% peptone, 0.045% CaCl2, 0.5% glucose, 0.15% K2HPO4, 0.05% KH2PO4, 0.05% NaCl, 0.2% Tween 80 and 44 mg/L diethylstilbestrol. The optimal culture conditions were as follows: initial pH 7.5, inoculum age 72 h, rotation rate 140 r/min, culture temperature 28 °C, culture time 72 h. [Conclusion] Under the optimal conditions, the degradation rate of diethylstilbestrol was 83.89%, 1.38-fold over the value before optimization (60.98%), showing a significant difference (p<0.01).

Key words:Diethylstilbestrol, Aspergillus oryzae, Degradation, Optimization