Abstract:Conjugation is the key and difficult point in microbiology teaching. For student’s intuitive understanding of conjugation, we set up a conjugating experiment using Escherichia coli and Shewanella oneidensis, on the basis of systematic introduction of its discovery procedure and molecular mechanisms. A problem oriented strategy was adopted in theory teaching, thereby student’s logic thinking was developed in questions, and the scientific rigor was imparted in discussions. An inquiry based method was applied in lab teaching, where student’s meticulous operating style was nourished via exploring conjugation efficiency under different environmental conditions. By this way, student’s flexible thinking abilities, questioning spirits, and broad humanistic wisdoms were cultivated, and their comprehensive innovation qualities were improved.