基于PCR-DGGE和高通量测序分析白云边酒窖泥细菌群落结构与多样性

Bacterial community and diversity in pit mud of Baiyunbian liquor analyzed by PCR-DGGE and high-throughput sequencing

摘要:

【目的】探索白云边酒不同窖龄窖泥细菌群落结构及其多样性，分析窖泥细菌群落特征对兼香型白酒风格形成的影响。【方法】分别提取2年和23年窖泥样品总DNA，采用PCR-DGGE、基因克隆以及高通量测序技术，研究窖泥细菌的分布情况。【结果】白云边窖泥细菌归属于变形菌门(Proteobacteria)、拟杆菌门(Bacteroidetes)、厚壁菌门(Firmicutes)、放线菌门(Actinobacteria) 4个菌门。2、23年窖泥共同的优势菌属(≥1.0%)包括棒状杆菌属(Corynebacterium)、香味菌属(Myroides)、鞘氨醇杆菌属(Sphingobacterium)、乳杆菌属(Lactobacillus)、梭菌属(Clostridium)、醋杆菌属(Acetobacter)、产碱杆菌属(Alcaligenes)、肠杆菌属(Enterobacter)和不动杆菌属(Acinetobacter)。2年窖泥特有优势菌属为Dysgonomonas、Fluviicola、变形杆菌属(Proteus)和Wohlfahrtiimonas，而23年窖泥特有优势菌属为葡萄球菌属(Staphylococcus)、Hazenella、魏斯氏菌属(Weissella)、葡糖醋杆菌属(Gluconacetobacter)和摩根氏菌属(Morganella)。【结论】2年窖泥细菌群落多样性高于23年窖泥。比较了白云边两种窖龄窖泥主要细菌组成情况，为研究微生物对白云边酒浓酱兼香型独特风味形成的影响提供依据。

Abstract:

[Objective] To study bacterial community and diversity in pit mud with different-aged cellars used to produce Baiyunbian liquor. [Methods] Total DNA of microbes in pit mud (2 and 23 year) were extracted, then PCR-DGGE and high-throughput sequencing technology were used to analyze bacterial communities. [Results] Bacteria in the pit mud fell into 4 phyla: Proteobacteria, Bacteroidetes, Firmicutes and Actinobacteria, with dominant genera (≥1.0%) including Corynebacterium, Myroides, Sphingobacterium, Lactobacillus, Clostridium, Acetobacter, Alcaligenes, Enterobacter, and Acinetobacter. Furthermore, Dysgonomonas, Fluviicola, Proteus and Wohlfahrtiimonas were present in 2-year uniquely, whereas Staphylococcus, Hazenella, Weissella, Gluconacetobacter and Morganella were especially predominant in 23-year pit mud. [Conclusion] Bacterial diversity in pit mud of 2-year was higher than in that of 23-year.