裂褶菌cfcc7252菌株对孔雀石绿染料的高效降解

Degradation of malachite green dye by Schizophyllum commune cfcc7252

摘要:

【目的】评价裂褶菌cfcc7252菌株降解孔雀石绿(Malachite Green，MG)的能力及其潜在的应用价值。【方法】采用单因子液体培养实验，研究了通气、pH、温度、碳源和氮源种类及浓度、金属离子、盐度、染料浓度对该菌降解效果的影响；采用平皿培养实验，利用植物种子萌发和微生物抑菌实验对降解产物进行毒性测试。【结果】研究结果表明，裂褶菌cfcc7252菌株在好氧和厌氧条件下均能高效降解MG。该菌在10.0 g/L葡萄糖，5.0 g/L酵母浸粉，0.01 mmol/L Zn2+，pH为4.0的液体培养基中培养36 h，对350 mg/L的MG降解率达67.8%；连续降解7次后，其降解率还能保持在95.4%以上。此外，该菌在盐度低于10.20%时，其对MG的降解率均达到98%以上。对植物、微生物的毒性测试结果表明，MG降解产物对红豆、豌豆等植物、金黄色葡萄球菌、枯草芽孢杆菌和铜绿假单胞杆菌等微生物基本没有毒性。【结论】裂褶菌cfcc7252菌株在处理以MG为主的染料废水时具有很强的应用潜力。

Abstract:

[Objective] To evaluate the ability of Schizophyllum commune cfcc7252 in degrading Malachite Green (MG) dye. [Methods] Effect of oxygen demand, initial pH value, temperature, carbon source, nitrogen source, salinity and initial dye concentration on MG biodegradation by S. commune cfcc7252 was studied in flasks containing liquid cultures based on single factor experiment design. The toxicity of the biodegraded products of MG on plant seed germination and microbial growth was determined in petri dishes. [Results] Schizophyllum commune cfcc7252 could degrade MG under both aerobic and anaerobic conditions; 67.8% MG (initial concentration: 350 mg/L) was degraded under the culture condition of 10.0 g/L glucose, 5.0 g/L yeast extract, 0.01 mmol/L Zn2+ and pH 4.0 for 36 h. After continuous bleaching for 7 rounds, the degradation rate still reached over 95.4%. In addition, the degradation rate of S. commune cfcc7252 on MG was as high as 98% if the salinity of the culture was less than 10.20%. Toxicity test showed that the biodegraded products of MG were rarely toxic to plants of Vigna umbellate and Vigna unguiculata and microorganisms of Staphylococcus aureus, Bacillus subtilis, Pseudomonas aeruginosa. [Conclusion] Schizophyllum commune cfcc7252 has strong potential to treat MG contaminated wastewater.