苎麻脱胶菌群RAMCD407中优势菌的分离、鉴定及脱胶能力分析

Isolation, identification and degumming efficiency of dominant bacteria from ramie degumming bacterial consortium RAMCD407

摘要:

【目的】以苎麻生物脱胶菌群RAMCD407为研究材料，分析其菌种功能，初步阐明菌群的种间协作机理。【方法】利用4种不同的培养基，对该菌群中的微生物进行分离培养，通过16S rRNA基因序列比对鉴定，将分离得到的菌株进行果胶酶活力、木聚糖酶活力和胶质去除率的测定与筛选。筛选得到的菌株重新组合成为复合菌系JHY，并分析各菌种对JHY的功能影响。【结果】共获得25个菌株，这25个菌株分别属于芽孢杆菌属、假单胞菌属、肠杆菌属、鲁梅利杆菌属、鞘氨醇杆菌属和微小杆菌属。从中筛选出6株细菌，分别为Y1、2H3、Y2、JY31、2H2和2H1，组成复合菌系JHY。经测定，2H2在复合菌系JHY中发挥重要作用，能提高复合菌系的果胶酶活力、木聚糖酶活力和胶质去除率；JY31的存在抑制了其它菌株的生长，降低了复合菌系JHY的酶活力和胶质去除率。【结论】2H2为复合菌系JHY的重要菌种，去除JY31可提高复合菌系JHY的脱胶效率。

Abstract:

[Objective] Ramie degumming bacterial consortium RAMCD407 was used to analyze the bacterial function and reveal the mechanism of interactions among different strains. [Methods] Several bacteria were cultured and isolated from RAMCD407 from four media, and these bacteria were then identified by 16S rRNA gene sequencing. Pectinase activity, xylanase activity and gum removal ratio of the isolated microbes were measured. Composite strains JHY were constructed by combination of the isolated strains, and the influences of different strains on the performance of JHY were analyzed. [Results] Twenty-five isolated strains were identified as genus Bacillus, Pseudomonas, Enterobacter, Rummeliibacillus, Sphingobacterium, and Exiguobacterium. Among them, strain 2H2 played an important role, and it could improve the pectinase activity, xylanase activity and gum removal ratio of JHY; However, strain JY31 could reduce the enzyme activity and gum removal ratio of JHY by inhibiting the growth of other strains. [Conclusion] 2H2 was the important strain in JHY, and elimination of JY31 could improve the gum removal efficiency of JHY.