第三代测序技术在微生物研究中的应用

Application of third generation sequencing technology to microbial research

摘要:

1977年Sanger发明的双末端终止法开启了测序之旅，而测序技术在30多年内不断革新。每种新技术的出现都有超过前代产品的独特之处，但也会不可避免的存在自身局限性，关键在于掌握每种技术的优缺点并加以合理应用。第三代测序技术是一种集高通量、快速度、长读长及低成本等多种优点于一身的新型测序技术，它的出现为基因组学、转录组学及DNA甲基化等研究注入了新活力。本文在介绍基本技术原理的基础上，着重概述了第三代测序技术在微生物研究中的应用，从而揭示了其广泛的应用前景。

Abstract:

The dideoxy chain-termination method, which was invented in 1977 by Sanger, has opened the door of DNA sequencing. The sequencing technology has continuously advanced during the past 30 years. Each new technology has unique advantages over the previous generation, but it also has its own limitations. The key is to recognize well both the advantages and disadvantages of each technology so that it can be used reasonably. The third generation sequencing technology is a new technology with many advantages, such as high throughput, fast speed, long read and low cost. Additionally, its emergence has advanced the study of genomics, transcriptomics, epigenomics and so on. In this review, the principle of the third generation sequencing technology is introduced, followed by a summary of its application to microbial research, and its potential uses in other areas.