紫外线和硫酸二乙酯复合诱变选育PHB高产菌株

Mutagenesis of poly-β-hydroxybutyricacid producing strain by UV and diethyl sulfate

摘要:

【目的】获得高产聚β-羟基丁酸酯(Poly-β-hydroxybutyricacid，PHB)菌株。【方法】以假单胞菌属Pseudomonas koreensis PK3菌株为出发菌株，采用硫酸二乙酯和紫外线相结合的诱变方法进行多轮诱变。【结果】经过初筛和复筛得到一株高产PHB突变菌株，命名为Pseudomonas koreensis UVCN-18。连续传代9次后，发酵28 h条件下，PHB产量达到15.94 g/L，占细胞干重69.54%，较原出发菌株Pseudomonas koreensis PK3 (4.42 g/L)提高了2.61倍，并且该菌株具有良好的遗传稳定性。【结论】采用硫酸二乙酯和紫外线相结合的诱变方法，成功获得了一株高产PHB突变菌株。

Abstract:

[Objective] We aim to get a high-yield poly-β-hydroxybutyricacid (PHB) mutant strain. [Methods] We use composite multiple rounds of mutagenesis breeding of UV and diethyl sulfate and after primary and secondary screening from the original strain Pseudomonas koreensis PK3. [Results] It’s named Pseudomonas koreensis UVCN-18. After serially passed 9 times and fermentation 28 h, PHB production of the mutant was 15.94 g/L, Accounting for cell dry weight 69.54%. PHB production of the mutant was increased by 2.61 times compared with the original strain Pseudomonas koreensis PK3 (4.42 g/L), and the strain has a good genetic stability. [Conclusion] We use composite multiple rounds of mutagenesis breeding of UV and diethyl sulfate to get a high-yield PHB mutant strain.