[Objective] To analyze lysine biosynthetic pathway and related genes based on the whole genome of Flammulina velutipes. [Methods] Genome sequence of the monosporous strain Dan3 was sequenced through Illumina Hiseq2000 and Roche454 FLX+. Key genes related to lysine biosynthesis pathway were screened based on the whole genome. Basic physical and chemical properties of these proteins were analyzed, and the subcellular localization and secondary structure were predicted. [Results] The sequenced reads were assembled and 34.17 Mb length genome sequence of Dan3 was obtained. The structure of 8 key genes involved in α-aminoadipate pathway was identified. Each gene was composed of a number of introns and exons, and the secondary structure was mainly composed of α-helix and irregular curl. Three of these proteins were localized to the mitochondria. [Conclusion] The de novo biosynthesis of lysine in F. velutipes was through the α-aminoadipate pathway, and almost all enzymes involved in this pathway were predicted in the whole genome of F. velutipes.
