中国大陆地区丛枝菌根真菌菌种资源的分离鉴定与形态学特征

Isolation and identification of arbuscular mycorrhizal fungi from Mainland China

摘要:

【目的】分离收集保藏中国大陆各个地区不同生态环境的丛枝菌根真菌菌种资源，为丛枝菌根的研究提供资源、奠定基础。【方法】以高粱为宿主植物，采用诱导培养、单孢培养和扩繁培养分离土壤样品中的丛枝菌根真菌菌种并鉴定。【结果】从我国大陆的45个地区50余种宿主植物根区土壤中分离到丛枝菌根真菌135株，隶属于23个种；对各个菌株的形态特征进行了描述。【结论】我国蕴藏着丰富的丛枝菌根真菌菌种资源，文中描述的菌种资源是目前从我国大陆地区获得的种类和数量最多、覆盖范围最广的AM真菌菌种资源。

Abstract:

[Objective] The aim of this study is to isolate the arbuscular mycorrhizal fungi (AMF) from different ecological environments in China, and lay the foundation for the research of AMF. [Methods] Relying on sorghum as the host plant, AMF strains were isolated from the soil using the trap cultures, single species cultures and stimulation of sporulation technologies respectively, followed by the species identification. [Results] One hundred and thirty-five AMF strains belonging to 23 species were isolated from the rhizosphere soil of over 50 host plants in 45 regions of China, and their morphological characteristics were described. [Conclusion] China is rich in the arbuscular mycorrhizal fungi resource, and the species resource.