3种野生牛肝菌的菌塘土壤细菌多样性

Bacteria diversity of shiroes soil growing three species of wild Boletus

摘要:

【目的】以远东疣柄牛肝菌(Leccinum extremiorientale)、美味牛肝菌(Boletus edulis)和小孢粉孢牛肝菌(Tylopilus microsporus)菌塘为研究对象，揭示其菌塘土壤细菌的菌群多样性。【方法】通过高通量测序方法，分析这3种野生牛肝菌菌塘土壤细菌的菌群结构及多样性。【结果】远东疣柄牛肝菌菌塘土样中的主要细菌类群为变形杆菌门(53.6%)、酸杆菌门(33.8%)、拟杆菌门(4.7%)、放线菌门(1.7%)；美味牛肝菌中主要类群为酸杆菌门(40.7%)、变形杆菌门(38.1%)、拟杆菌门(10.3%)、放线菌门(3.1%)；小孢粉孢牛肝菌中酸杆菌门(65.3%)、变形杆菌门(23.8%)、拟杆菌门(4.9%)为主要类群。细菌在属级分类水平上，远东疣柄牛肝菌菌塘主要属中69.8%为未知属，其余为伯克氏菌属Burkholderia (16.0%)、酸杆菌门中的Candidatus Solibacter (5.9%)和酸孢菌属Acidocella (3.3%)。美味牛肝菌中主要的属分布在未知类群(83.0%)，此外，还有Candidatus Solibacter (8.0%)，伯克氏菌属Burkholderia (2.1%)。小孢粉孢牛肝菌菌塘中，分别为未知类群(80.9%)和Candidatus Solibacter (12.6%)。【结论】3种牛肝菌菌塘土壤中细菌类群具有丰富的多样性，其中含有大量未知类群。

Abstract:

[Objective] In order to study the community diversity of shiroes soil, shiroes of Leccinum extremiorientale (Le), Boletus edulis (Be) and Tylopilus microsporus (Tm) were investigated. [Methods] Bacterial diversity and community structure of shiroes of these wild Boletus were analyzed via high throughput sequencing. [Results] Major phyla of Le, Be and Tm were Proteobacteria (53.6%), Acidobacteria (33.8%), Bacteroidetes (4.7%), Actinobacteria (1.7%); Acidobacteria (40.7%), Proteobacteria (38.1%), Bacteroidetes (10.3%); Acidobacteria (65.3%), Proteobacteria (23.8%) and Bacteroidetes (4.9%), respectively. The dominant genera of Le, Be and Tm were unclassified bacteria (69.8%), Burkholderia (16.0%), Candidatus Solibacter (5.9%), Acidocella (3.3%); unclassified bacteria (83.0%), Candidatus Solibacter (8.0%), Burkholderia (2.1%); unclassified bacteria (80.9%), Candidatus Solibacter (12.6%), respectively. [Conclusion] The bacterial diversity is abundant in the three shiroes and there are a number of unclassified bacteria.