响应面设计优化绿僵菌固体发酵条件

Metarhizium solid-state fermentation optimization by response surface analysis

摘要:

【目的】为了提高绿僵菌分生孢子产量及孢子质量，应用响应面设计对金龟子绿僵菌菌株CY-1 (Metarhizium anisopliae)进行固体发酵培养基的优化。【方法】单因素试验基础上，采用响应面试验设计方法优化培养基组分。【结果】添加了碳、氮营养的最佳固体发酵培养基为玉米粉:稻壳=8:2，料水比1:0.8，葡萄糖0.8%，硫酸铵2.5%，磷酸二氢钾0.8%；在固体培养基上的理论产孢量为7.45×109个/g；验证后实际为6.94×109个/g。【结论】运用响应面法对绿僵菌固体发酵的培养基成分进行优化，得到了绿僵菌孢子粉，为孢子粉进行地下害虫防治和制剂加工的研究奠定了基础。

Abstract:

[Objective] In order to improve the yield and quality of the Metarhizium conidia spores, response surface design was used to optimize the medium components for solid fermentation of the Metarhizium anisopliae CY-1. [Methods] On the basis of one-factor experiment, medium components were optimized by the response surface design. [Results] The carbon and nitrogen sources were added to the best solid fermentation medium, which also contained cornmeal and rice husk (8:2, W/W), water ratio 1:0.8, 0.8% glucose, 2.5% ammonium sulfate, 0.8% KH2PO4. In the optimized solid fermentation medium, the theory of spores production was 7.45×109 CFU/g, and the validation was actually 6.94×109 CFU/g. [Conclusion] the response surface method was applied to optimize the components of solid fermentation medium of the M. anisopliae, and the spores powders were obtained, which laid a foundation for the research of the control the underground soil borne pathogens and insect pests and the research of the powder preparation and processing.