[Objective] The objectives were to study and discover thermophilic fungi from soil in western China. [Methods] Different environmental and regional soil samples were collected and cultivated by the enrichment culture using feather. The strains of the genus Myceliophthora were isolated and identified by the classical morphological characters and molecular systematics. [Results] Strain G10 was identified as Myceliophthora heterothallica; EB6301M was M. vellerea and other ten strains were M. thermophile. [Conclusion] M. heterothallica and M. vellerea were two new record species in China.