3种淡水环境中低核酸含量细菌的滤过性

Filterability of bacteria with low nucleic acid content in three freshwater environments

摘要:

【目的】增加低核酸含量(LNA)细菌与过滤性细菌之间的认识。【方法】采用流式细胞技术(FCM)、变性梯度凝胶电泳(DGGE)及统计学分析研究3种典型淡水环境中细菌群落与滤过性。【结果】LNA细菌与过滤性细菌在数量上具有很好的相关性(y=0.646x+22.42，R2=0.984，P<0.01)，细菌的滤过性不仅与细菌大小有关，还与细胞整体形状及其伸缩性有关；细菌群落组织与LNA细菌比重呈负相关性，而与HNA细菌呈正相关性。【结论】0.45 μm膜过滤对水样微生物群落中的LNA细菌具有极强的筛选效果；细菌群落组织与其基于流式细胞技术测定的基因含量具有密切联系。

Abstract:

[Objective] We studied the correlation between low nucleic acid content (LNA) bacteria and their filterability. [Methods] Bacterial community and filterability in three freshwater environments were analyzed by flow cytometry (FCM), denaturing gradient gel electrophoresis (DGGE) and statistical analysis. [Results] The proportion of LNA bacteria and their filterability had a significant correlation (y=0.646x+22.42, R2=0.984, P<0.01), and the filterability of LNA bacteria depended on not only the cell size, but also shape and flexibility. In addition, bacteria community organization positively correlated to the proportion of high nucleic acid content (HNA) bacteria and negative correlated to the proportion of LNA bacteria. [Conclusion] The results indicated that 0.45 μm pore-size-filtration can efficiently screen LNA bacteria from the autochthonous microbial community, and demonstrated that bacterial community organization was closely related to the nucleic acid content measured by flow cytometry.