黄芩苷对阪崎克罗诺杆菌生物膜的抑制作用

Inhibition of Cronobacter sakazakii biofilms by baicalin

摘要:

【目的】分析黄芩苷对阪崎克罗诺杆菌生物膜的抑制作用。【方法】采用XTT法评价黄芩苷对阪崎克罗诺杆菌起始粘附性及生物膜内细菌细胞活性的影响，并且采用荧光实时定量PCR (Quantitative real-time PCR，qRT-PCR)检测了阪崎克罗诺杆菌生物膜相关基因glpQ、nlpD、gsiB、deoB、luxS、sdiA的表达水平。【结果】黄芩苷对阪崎克罗诺杆菌的抑制效果呈剂量依赖型。黄芩苷对阪崎克罗诺杆菌的MIC80值为1 024 mg/L，该浓度的黄芩苷对阪崎克罗诺杆菌BAA-894和IQCC10423菌株生物膜的抑制率分别为83.7%和53.2%。浓度为2 048 mg/L的黄芩苷能够通过降低阪崎克罗诺杆菌的粘附性来抑制新生物膜的形成。另外，实时定量PCR结果表明黄芩苷可能通过下调阪崎克罗诺杆菌生物膜相关基因的表达来抑制其生物膜的形成。【结论】黄芩苷有可能被作为抗菌剂以预防和灭活阪崎克罗诺杆菌生物膜。

Abstract:

[Objective] We studied the inhibition of Cronobacter sakazakii biofilm by baicalin. [Methods] XTT reduction assay was used to evaluate the effects of baicalin on the early adhesion of C. sakazakii planktonic cells and the activity of sessile cells in biofilms. Quantitative real-time PCR (qRT-PCR) was used to determine the expression level of the biofilm-associated genes, including glpQ, nlpD, gsiB, deoB, luxS, and sdiA. [Results] The inhibition of C. sakazakii biofilms by baicalin was dose dependent. MIC80 of baicalin against C. sakazakii was 1 024 mg/L, with 83.7% inhibition of C. sakazakii ATCC BAA-894 and 53.2% of C. sakazakii IQCC10423. In addition, 2 048 mg/L of baicalin inhibited the adherence of C. sakazakii at its earlier development of new biofilms. qRT-PCR results demonstrated that baicalin could down-regulate the expression of biofilm-associated genes in C. sakazakii and further inhibited its biofilm formation. [Conclusion] Baicalin could be potentially used as an antimicrobial to inhibit C. sakazakii biofilms.