L-精氨酸生物合成机制及其代谢工程育种研究进展

Progress in biosynthesis and metabolic engineering of L-Arginine producer

摘要:

L-精氨酸是人体半必需的氨基酸，在生命代谢过程中起着非常重要的作用，且具有广泛的应用价值及市场需求。目前，L-精氨酸主要采用微生物发酵法进行生产，为了提高L-精氨酸的产量和稳定性，最有效的方法是优化L-精氨酸生产菌株，通过代谢工程改造微生物菌株有望达到这一目的。本文分析了微生物中L-精氨酸的代谢途径和调控机制，并综述了构建高产L-精氨酸的代谢工程策略。此外，展望了菌株稳定性和底物扩展利用的未来研究方向。

Abstract:

L-Arginine, a semi-essential amino acid, plays an important role in the metabolism of human life. It has a wide and expanding application in industry with a fast growing market demand. Currently, the main method of production of L-Arginine is microbial fermentation. To develop microbial strains with high productivity and stability, the most effective method is to optimize the L-Arginine producing strains. And fortunately, metabolic engineering method can be used to screen the efficient L-Arginine producing strains, and has obtained the certain success. In this mini-review, the metabolic pathway and regulatory mechanism of L-Arginine biosynthesis in microorganisms were analyzed, and the strategies for metabolic engineering the efficient L-Arginine producing strains were summarized．Furthermore, the prospects of future research about the stability of L-Arginine engineering strains and the expansions of substrate utilization were discussed.