16s rRNA基因克隆文库分析比较牙周炎患者和健康人口腔唾液微生物多样性

Comparison of oral microbial diversity between periodontitis patients and healthy people by 16S rRNA gene clone library

摘要:

【目的】通过对同一地区、同一民族牙周炎患者和健康人的唾液微生物群落结构的分析，探寻牙周炎患者口腔微生物的多样性。【方法】采集甘肃东乡族自治县的东乡族牙周炎患者和健康人唾液各5例，分别记作DP (东乡牙周)和DH (东乡健康)，提取细菌总DNA，构建16S rRNA基因克隆文库，测序后利用mothur、MEGA 4.0、ClustalX 3.0等软件对测序结果进行分析。【结果】所有样本共检测出115个OTUs (DP 60，DH 75)，归属于6个门，27个属。TM7是DP组特有的优势菌门。仅在DP组中检测到的优势菌属是梭菌属(Fusobacterium)、卟啉单胞菌属(Porphyromonas)、消化链球菌属(Peptostreptococcus)和TM7_genera。【结论】发现牙周炎患者与健康人口腔唾液微生物存在一定差异。其中，TM7、梭菌属和消化链球菌属在牙周病中的作用值得进一步研究。

Abstract:

[Objective] To compare oral microbial diversity between periodontitis patients and healthy people by analyzing the saliva microbial community structure in the same region with same nationality. [Methods] We collected 10 saliva samples from 10 subjects (5 from periodontitis patients, 5 from healthy people) and marked DP (Dongxiang periodontitis patients) and DH (Dongxiang healthy people). Saliva samples’ DNA was extracted and the 16S ribosomal RNA gene clone library was constructed. The sequencing results were analyzed by software such as MOTHUR, MEGA 4.0, and ClustalX 3.0. [Results] A total of 115 OTUs (DP 60; DH 75) were obtained and all OTUs were classified into 6 phyla and 27 genera. TM7 was the unique dominant phyla in DP group. The dominant genera in DP group only included: Fusobacterium, Porphyromonas, Peptostreptococcus, TM7_genera. [Conclusion] There is a certain difference of oral microbial community structure between Dongxiang periodontitis and healthy people. The roles of Fusobacterium, Peptostreptococcus, TM7_genera in oral cavity of periodontitis patients may deserve further study.