裂殖壶菌Schizochytrium sp. 20888产DHA培养条件优化

Optimization of cultivation conditions for DHA production by Schizochytrium sp. 20888

摘要:

【目的】为了优化裂殖壶菌产DHA的培养条件，提高油脂中DHA含量。【方法】采用单因素试验和正交设计试验方案，针对分批培养时间、培养基碳、氮源的种类和浓度以及培养温度开展试验，采用重量法测定生物量、采用索氏提取法测定油脂总量，采用气相色谱法测定油脂DHA含量，考察培养条件对细胞油脂DHA含量的影响。【结果】最适培养时间为4 d，培养温度23 °C，最优碳氮源组成为(g/L)：葡萄糖65、甘油80、蛋白胨6、酵母粉4和谷氨酸钠8。【结论】裂殖壶菌Schizochytrium sp. 20888在葡萄糖和甘油组成的复合碳源和由蛋白胨、酵母粉和谷氨酸钠组成的复合氮源的培养基中可以得到最优的DHA产量，细胞DHA含量能达到33.68%。

Abstract:

[Objective] In order to optimize the cultivation conditions of the Schizochytrium sp. 20888 to increase DHA yield. [Methods] The effects of cultivation time, carbon and nitrogen sources and their concentrations and culture temperature on the cell growth, cellular lipid and DHA contents were studied. A series of “one-factor-at-a-time” experiments and orthogonally designed tests were carried out. The biomass was tested as dried weight, the lipid content was tested by Soxhlet extraction and the DHA content was determined by gas chromatography. [Results] The optimal cultivation time was 4 d, the proper temperature was 23 °C, and the optimal medium formula was as following (g/L): glucose 65, glycerol 80, peptone 6, yeast extract 4, sodium glutamate 8. [Conclusion] Optimal cultivation conditions for DHA production were obtained successfully. Under the optimal conditions, the DHA yield was reached to 33.68%.