3株罗伊氏乳杆菌生物学特性的分析比较

Comparative analysis of the biological characteristics of three strains of Lactobacillus reuteri

摘要:

【目的】对3 株罗伊氏乳杆菌的生物学特性进行分析比较,为后期生产应用提供一定的参考。【方法】对实验室保藏的3 株罗伊氏乳杆菌的生长曲线、pH 曲线、耐受人工胃液能力、耐受猪胆盐能力、黏附能力、抑菌能力和对抗生素的耐药性等特性进行了分析比较。【结果】3 株菌生长趋势大致相同;3 株菌对人工胃液均具有良好的耐受性,且可以有效地抑制大肠杆菌和金黄色葡萄球菌的生长;菌株L0 和L2 对高胆盐的环境耐受性较差,菌株L1 则对高胆盐环境具有极强的耐受性;菌株L1 和L2 具有很强的黏附能力;3 株菌对20 种抗生素表现出不同的耐受性。【结论】菌株L1 的生物学特性明显优于其他两株菌株,有利于后期的生产应用。

Abstract:

[Objective] The study researched the biological characteristics of three Lactobacillus reuteri strains, providing guidance in industrial production. [Methods] We analyzed and compared diverse characteristics and capabilities of three Lactobacillus reuteri strains preserved in the lab, such as growth curve, pH curve, tolerance to artificial gastric intestinal juice, tolerance to porcine bile salt, adhesive capacity, antimicrobial activity, and tolerance to antibiotics. [Results] The growth trend of these 3 strains was similar. All of the 3 strains had a good tolerance to artificial gastric intestinal juice, and could inhibited the growth of Escherichia coli and Staphylococcus aureus effectively. L0 and L2 showed poor tolerance in high porcine bile salt environment, while the performance of L1 was satisfactory. In addition, L1 and L2 had strong adhesive ability and the tolerances of 3 strains to 20 antibiotics were different. [Conclusion] L1 can be further applied in the industrial production since its biological characteristics are significantly better than the other two strains.