不同光照强度对小球藻生长的影响

Effects of different light intensity on the growth of Chorella vulgaris

摘要:

【目的】优化小球藻的培养与提高资源化利用效率,得到不同光照强度对小球藻生长的影响规律。【方法】通过室内控制实验,设置0、1 000、3 000、5 500、7 000、9 000 lx 共6 组光照强度,在温度为20 ℃ 和曝气强度为20%的条件下,采用BG-11 培养基培养小球藻(Chorella vulgaris)至稳定生长,研究不同光照强度对小球藻生长的影响,建立光照强度与最大光密度OD_max、最大比增长率μ_max 之间的抛物回归曲线。【结果】不同光照强度下,小球藻生长差异较大,培养初期叶绿素a 浓度迅速增加直至峰值,后期迅速降低,其中5 500 lx 强度下小球藻生长最好。藻类生长对溶解性总磷的消耗比溶解性总氮更大。【结论】光照强度(x)与OD_max 的拟合方程为:OD_max=-1E–07x²+0.001 6x+0.105 5 (R²=0.987 2,0≤x≤9 000 lx),光照强度(x)与μ_max 的拟合方程为:μ_max=-2E–08x²+0.000 3x-0.004 2 (R²=0.998 6,0≤x≤9 000 lx)。

Abstract:

[Objective] This study provides the theoretical basis for optimal cultivation and nutrient utilization of Chorella vulgaris. The study aimed to discuss the effect of varying light intensity (0, 1 000, 3 000, 5 500, 7 000, 9 000 lx) on the growth of Chorella vulgaris. [Methods] The experiment was conducted under aeration rate of 20% and at 20 ℃, and the Chorella vulgaris was cultivated on the BG-11 culture medium under stable condition. Parabolic regression curves between light intensity and the maximum optical density (OD_max) and the maximum growth rate (μ_max) were created. [Results] The growth rates varied different in different light intensity, and the chlorophyll a concentrations increased in the initial time until reached the maximum value and then decreased dramatically. The intensity of 5 500 lx was most suitable for the Chorella vulgaris growth. The demand of phosphorus of algal growth was greater than the nitrogen. [Conclusion] The mathematical relationships between light intensity and OD_max, μ_max simulated by cubic curve equation were shown as follows: OD_max=-1E–07x²+0.001 6x+0.105 5 (R²=0.9872, 0≤x≤9 000 lx), μ_max=-2E–08x²+0.000 3x-0.004 2 (R²=0.998 6, 0≤x≤9 000 lx).