内蒙古呼伦贝尔地区传统发酵乳中乳酸菌的多样性分析

Diversity of lactic acid bacteria associated with traditional fermented milk from Hulun Buir in Inner Mongolia

摘要:

【目的】对内蒙古呼伦贝尔地区传统发酵乳制品中乳酸菌资源的生物多样性进行研究。【方法】采用纯培养和16S rRNA 基因序列分析法对内蒙古呼伦贝尔地区传统发酵乳中的乳酸菌进行多样性分析。【结果】从8 份传统发酵乳制品(6 份酸牛奶和2 份酸马奶)样品中分离到24 株乳酸菌,通过16S rRNA 基因序列分析和系统进化关系分析将24 株乳酸菌鉴定为2 株Lactobacillus kefiranofaciens、2 株Lactobacillus kefiri、5 株Lactobacillus paracasei、3 株Lactobacillus plantarum、1 株Lactobacillus rhamnosus、6 株Lactococcus lactis subsp. lactis、2 株Leuconostoc mesenteroides subsp. dextranicum、2 株Streptococcus thermophilus 和1 株Enterococcus faecium。【结论】Lactococcus lactis subsp. lactis 为内蒙古呼伦贝尔地区传统发酵乳制品的优势菌种,占总分离株的25%,其次为Lactobacillus paracasei,占总分离株的20.83%。

Abstract:

[Objective] The diversity of lactic acid bacteria in traditional fermented milk from Hulun Buir in Inner Mongolia was studied. [Methods] The samples were analyzed using pure culture and 16S rRNA gene sequence analysis. [Results] A total of 24 strains of lactic acid bacteria were obtained from eight fermented milk samples (6 fermented cow milk and 2 fermented mare milk). All the isolates were classified to 5 genera and 9 species: Lactobacillus kefiranofaciens (2 strains), Lactobacillus kefiri (2 strains), Lactobacillus paracasei (5 strains), Lactobacillus plantarum (3 strains), Lactobacillus rhamnosus (1 strain), Lactococcus lactis subsp. lactis (6 strains), Leuconostoc mesenteroides subsp. dextranicum (2 strains), Streptococcus thermophilus (2 strains), and Enterococcus faecium (1 strain). [Conclusion] Lactococcus lactis subsp. lactis is the dominant species, held 25% of the total isolated lactic acid bacteria, and Lactobacillus paracasei occupied 20.83% of the total lactic acid bacteria isolates as the predominant species of traditional fermented milk from Hulun Buir in Inner Mongolia.