一种寄生茶小绿叶蝉蜡蚧菌的鉴定及产孢条件优化

Identification of a parasitic Lecanicillium of tea lesser leafhopper and optimization of sporulation conditions

摘要:

【目的】对寄生茶小绿叶蝉的丝状真菌进行鉴定和分生孢子培养研究。【方法】采用形态特征比较和转录间隔区(ITS)序列构建系统树进行分析,分生孢子培养通过单因素筛选和正交试验进行产孢条件优化。【结果】根据形态特征比较和系统发育分析表明,该真菌为渐狭蜡蚧菌Lecanicillium attenuatum Zare & W. Gams。最优产孢条件(质量体积比)为:蛋白胨2%,麦芽糖1%,蚕蛹粉1%,氯化钾0.05%,磷酸氢二钾0.1%,七水硫酸镁0.05%,琼脂1.5%,蒸馏水1 000 mL,25 ℃。【结论】通过形态特征比较和分子序列分析表明,罹病茶小绿叶蝉上的真菌为已知种——渐狭蜡蚧菌,并对该菌株进行了产孢条件的优化。研究结果为该菌株应用到茶小绿叶蝉的生物防治研究提供基础资料。

Abstract:

[Objective] The objectives are to identify a parasitic Lecanicillium W. Gams & Zare of tea lesser leafhopper and optimize sporulation culture condition of this fungus. [Methods] The comparison of morphological characteristics and internal transcribed spacer (ITS) sequence analysis were used for identification of the fungal species. Single factor test and orthogonal test were carried out to optimize sporulation conditions. [Results] According to the morphological characterization and phylogenetic tree, the fungus was identified as Lecanicillium attenuatum Zare & W. Gams. The optimum condition of or sporulation is: peptone 2%, maltose 1%, silkworm pupa powder 1%, potassium chloride 0.05%, dipotassium phosphate 0.1%, magnesium sulfate 0.05%, agar 1.5%, distilled water 1 000 mL, 25 ℃. [Conclusion] A parasitic Lecanicillium of tea lesser leafhopper is identified as L. attenuatum Zare & W. Gams, a known species in China. The optimum condition of sporulation was providedons, which could provide the preliminary basis for applying to the tea lesser leafhopper of the biological control.