来源于中国南海的微生物新种乙醇速生杆菌(Celeribacter ethanolicus)多相分类研究

Celeribacter ethanolicus sp. nov., isolated from seawater of the South China Sea

摘要:

【目的】研究来源于南海海水的一株速生杆菌属菌株NH195^T 的多相分类。【方法】采用表型、基因型和化学分类方法,并综合系统发育关系结果,分析菌株NH195^T 的分类学地位。【结果】菌株NH195^T 是一株革兰氏阴性、好氧、杆状、无运动性细菌;能积累Poly-β-hydroxybutyrate (PHB);能在0.5%-10.0% (质量体积比) NaCl 浓度,pH 5.0-9.0 和20-40 ℃ 条件下生长,最适NaCl 生长浓度为1.0%-3.0%;氧化酶、触酶和脲酶反应结果阳性。菌株NH195^T 主要呼吸醌为Q-10,主要脂肪酸为C_18:1ω7c、C_18:1ω6c 和11 methyl C_18:1ω7c,主要极性脂为磷脂酰胆碱、磷脂酰甘油、双磷脂酰甘油、一个未知的氨基脂和两个未知脂。基因组G+C 含量为61.3 mol%。基于16S rRNA 基因的系统发育结果显示,菌株NH195^T 隶属于速生杆菌属;其与速生杆菌属标准菌株的16S rRNA 基因相似性范围为94.4%-97.7%。菌株NH195^T 与速生杆菌属标准菌株C. halophilus ZXM137^T 和C. indicus P73^T 的平均核苷酸一致性(ANI)分别为78.6%和78.0%;基于基因组数据计算所得的DNA 杂交同源率分别为26.1%和23.0%。【结论】基于表型和基因型结果,菌株NH195^T 代表了速生杆菌属一个新物种,命名为Celeribacter ethanolicus,标准菌株为NH195^T (CGMCC 1.15406^T=JCM 31095^T)。

Abstract:

[Objective] A novel Celeribacter strain NH195^T was isolated from seawater of the South China Sea and was subjected to polyphasic taxonomic study. [Methods] Phenotypic, genotypic and chemotaxonomic characterizations, as well as phylogenetic inference were performed in this study. [Results] Strain NH195^T was a Gram-stain-negative, aerobic, rod-shaped, non-motile bacterium that accumulates poly-β-hydroxybutyrate (PHB). The isolate grew in media containing 0.5%-10.0% (W/V) NaCl (optimally at 1.0%-3.0%) at 20-40 ℃ and pH 5.0-9.0, and was positive for oxidase, catalase and urease. The respiratory quinone was Q-10. The major fatty acids (>10%) were C_18:1ω7c, C_18:1ω6c and 11 methyl C_18:1ω7c. The major polar lipids were phosphatidylcholine (PC), phosphatidylglycerol (PG), diphosphatidylglycerol (DPG), one unidentified aminolipid and two unidentified lipids. The genomic DNA G+C content was 61.3 mol%. Phylogenetic analysis based on the 16S rRNA gene sequences indicated that strain NH195^T could be assigned to the genus Celeribacter. The 16S rRNA gene sequence similarities between the isolate and the type strains of Celeribacter species with validly published names were in the range 94.4%-97.7%. Strain NH195^T exhibited average nucleotide identity (ANI) values of 78.6% and 78.0% to C. halophilus ZXM137^T and C. indicus P73^T respectively. The genome-to-genome distance analysis revealed that strain NH195^T shared 26.1% DNA relatedness with C. halophilus ZXM137^T and 23.0% with C. indicus P73^T, respectively. [Conclusion] On the basis of phenotypic and genotypic characteristics, strain NH195^T represents a novel species within the genus Celeribacter, for which the name Celeribacter ethanolicus is proposed. The type strain is NH195^T (=CGMCC 1.15406^T=JCM 31095^T).