Abstract:At the beginning of the founding of college of environment and ecology, Xiamen University, we made great efforts on the establishment of biological and experimental courses for undergraduate of environmental science and ecology major. This article discusses the exploration and practice on developing “Environmental Microbiology Experiment” course for undergraduate of environmental science and ecology major. The discussion content in this paper includes the following aspects: curriculum setting, training of basic experimental skills and cultivation of scientific literacy, experimental curriculum assessment methods, teaching effect and evaluation. Students of environmental science and ecology major master the basic skills and comprehensive ability from basic experiments and inde-pendent design experiments.