Abstract:This article introduces the teaching reform practices of Microbiology by flipped classroom in Huazhong Agricultural University, Wuhan, China. By means of classroom teaching, homework, course thesis, topic discussion, online FAQ etc, students are actively guided to self-directed learning microbiology related knowledge. The flipped classroom has been in combination with the traditional classroom. And the “course teaching” has been shifted to “knowledge internalization”. Students not only master the microbiology related knowledge, but also can independently and actively study by the way of flipped classroom.